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In order to understand its function and what it is, one must understand the concept behind the protein/peptide structure of these hormones (which will also consequently help the reader to further understand the difference in the naming that has been discussed above). Chemical Characteristics of Modified GRF 1-29 (CJC-1295 without DAC) Proteins/peptides are chains of amino acids linked in specific unique orders. Proteins, depending on how many amino acids it is composed of, will manifest itself as one of three structural types: the primary protein structure which is simply a long chain of amino acids, the secondary protein structure which is a folded protein structure resembling a pleated sheet, the tertiary protein structure which is a complex fold of proteins that resembles a tangled ball of yarn, and finally the quaternary protein structure which is several tertiary proteins interconnected. The significance of this in protein hormones such as Human Growth Hormone (HGH), insulin, Luteinizing Hormone (LH), Mod GRF 1-29 (CJC-1295 without DAC), and many others, is the fact that various sections of the protein structure will contribute to different jobs in the body by binding to and activating different receptors. The shape of the peptide hormone, and the different subsections of it, can allow stronger binding affinity or weaker binding affinity. Growth Hormone Releasing Hormone (GHRH), which is the endogenously secreted GHRH by the arcuate nucleus of the hypothalamus of the human body, is 44 amino acids long. It was discovered, however, that only the first 29 amino acids of the protein were equally as effective in binding to receptors on the pituitary gland as the full 44 amino acid structure[ii] [iii]. The first 29 amino acids in its protein structure were then isolated, which was then called GRF 1-29, but the problem with this derivative of GHRH was the fact that it was rapidly metabolized and cleared from the body by enzymes. Studies have reported that GRF 1-29’s half-life is less than 10 minutes and as little as 5 minutes[iv]. This was obviously not enough time to ensure a maximized and sustained release of HGH from the pituitary gland, as studies have demonstrated that the full potential of an HGH pulse from the pituitary requires at least 30 minutes as evidenced by the fact that much higher HGH levels (50 times greater) were observed 15 ‘ 30 minutes into subcutaneous administration of GHRH analogues[v], eq 300 mg injectable steroids $58.00 equipoise, eq. Therefore, the solution to this was to modify GRF 1-29 by replacing various amino acids in its structure with other amino acids that would provide a greater resistance to breakdown and cleavage by enzymes. There were many modified analogues developed, and Mod GRF 1-29 (CJC-1295 without DAC) was eventually selected for use, which demonstrated the most promising effects. Mod GRF 1-29 is a modification of GRF 1-29, specifically at amino acids #2, #8, #15, and #27. The result is an extended half-life to that of at least 30 minutes[vi] [vii]. Properties of Modified GRF 1-29 (CJC-1295 without DAC) Mod GRF 1-29 acts upon receptors located in the anterior pituitary gland, and signals the pituitary gland to increase Human Growth Hormone production and cause a release of massive quantities of Human Growth Hormone in a pulsatile manner. The effects of Mod GRF 1-29 are very similar from what would be expected from synthetic HGH administration over the long term (see the Human Growth Hormone profile here), although the amount of time that the released human growth hormone will remain in circulation is of a far less amount of time than synthetic Human Growth Hormone does. Therefore, multiple applications of Mod GRF 1-29 is recommended throughout the day in order to simulate Human Growth Hormone levels that remain high on a constant basis. Mod GRF 1-29 (CJC-1295 without DAC) is commonly combined with a Ghrelin mimetic (also known as a GHRP ‘ Growth Hormone Releasing Hexapeptide), such as GHRP-6, GHRP-2, Hexarelin, or Ipamorelin in order to initiate and amplify a greater pulse of HGH from the pituitary compared to Mod GRF 1-29 used solitarily on its own. The effects of a GHRH analogue (such as Mod GRF 1-29) and a Ghrelin mimetic (a GHRP such as GHRP-6 or Ipamorelin) are synergistic and amplify the release of HGH from the pi. The side effects associated with Mod GRF 1-29 can all be attributed to Human Growth Hormone’s side effects, seeing as though the actual specific end result of Mod GRF 1-29 (CJC-1295 without DAC) is to achieve vast increases of naturally occurring endogenously produced Human Growth Hormone. 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